Difference between revisions of "Part:BBa K200024"
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RcsB overexpression turn on the RCS phosphorelay system. This results in the modulation of ~5% of the <i>E.coli</i> genome. One of the most notable effects of this is the production of the exopolysaccharide colanic acid. | RcsB overexpression turn on the RCS phosphorelay system. This results in the modulation of ~5% of the <i>E.coli</i> genome. One of the most notable effects of this is the production of the exopolysaccharide colanic acid. | ||
| − | The double terminator is the most commonly used terminator and is a combination of parts BBa_B0010 and BBa_B0012. | + | The double terminator is the most commonly used terminator and is a combination of parts ([https://parts.igem.org/Part:BBa_B0010 Design BBa_B0010]) and ([https://parts.igem.org/Part:BBa_B0012 Design BBa_B0012]). |
The double terminator allows the RcsB gene to be incorporated into a closed transcriptional unit. | The double terminator allows the RcsB gene to be incorporated into a closed transcriptional unit. | ||
Revision as of 16:06, 18 October 2009
RcsB+TT
Colanic acid producing gene with double terminator
RcsB (BBa_K200000) coupled to the double terminator (BBa_B0015).
RcsB overexpression turn on the RCS phosphorelay system. This results in the modulation of ~5% of the E.coli genome. One of the most notable effects of this is the production of the exopolysaccharide colanic acid.
The double terminator is the most commonly used terminator and is a combination of parts (Design BBa_B0010) and (Design BBa_B0012).
The double terminator allows the RcsB gene to be incorporated into a closed transcriptional unit.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]