Difference between revisions of "Part:BBa K200024"
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Colanic acid producing gene with double terminator
 
Colanic acid producing gene with double terminator
  
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RcsB (BBa_K200000) coupled to the double terminator (BBa_B0015).  
+
RcsB ([https://parts.igem.org/Part:BBa_K200000:Design BBa_K200000]) coupled to the double terminator ([https://parts.igem.org/Part:BBa_B0015:Design BBa_B0015]).
  
  

Revision as of 16:04, 18 October 2009

RcsB+TT

Colanic acid producing gene with double terminator

RcsB (BBa_K200000) coupled to the double terminator (BBa_B0015).


RcsB overexpression turn on the RCS phosphorelay system. This results in the modulation of ~5% of the E.coli genome. One of the most notable effects of this is the production of the exopolysaccharide colanic acid.

The double terminator is the most commonly used terminator and is a combination of parts BBa_B0010 and BBa_B0012.

The double terminator allows the RcsB gene to be incorporated into a closed transcriptional unit.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]