Difference between revisions of "Part:BBa K4604018:Design"
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===Design Notes===
 
===Design Notes===
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Site directed mutagenesis was done between the tet promoter and RBS to remove a EcoRI restriction site.  
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Site directed mutagenesis was done between the tet promoter and RBS to remove a EcoRI restriction site. The region downstream of the tetA/R promoter was shortened (in comparison to piG_01a/BBa_K4604016) to counteract the leakiness.  
  
  

Revision as of 15:54, 7 October 2023


piG_02b (tetR_riboK12_mazF_mTurq)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 710
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 883
    Illegal AgeI site found at 1376
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2250


Design Notes

Site directed mutagenesis was done between the tet promoter and RBS to remove a EcoRI restriction site. The region downstream of the tetA/R promoter was shortened (in comparison to piG_01a/BBa_K4604016) to counteract the leakiness.


Source

Modified piG_02a (BBBa_K4604017) using Gibson Assembly.

References