Difference between revisions of "Part:BBa K4604015:Design"
Line 15: Line 15:
 
===Source===
 
===Source===
  
−
Modified piG_01a using Gibson Assembly.  
+
Modified piG_01a (BBa_K4604016) using Gibson Assembly.  
  
 
===References===
 
===References===

Revision as of 15:54, 7 October 2023


piG_01b (tetR_bluB)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 710
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1618


Design Notes

The start codon of the bluB was changed to ATG to act as a start for translation in E. coli. The region downstream of the tetA/R promoter was shortened (in comparison to piG_01a/BBa_K4604016) to counteract the leakiness.



Source

Modified piG_01a (BBa_K4604016) using Gibson Assembly.

References