Difference between revisions of "Part:BBa K4727113"

 
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<partinfo>BBa_K4727113 short</partinfo>
 
<partinfo>BBa_K4727113 short</partinfo>
  
This guide DNA has been designed to work with dCas9 to target AMR related genes in
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This guide DNA has been designed to work with dCas9 to target AMR related genes in <i>Klebsiella pneumoniae</i>. The guide binds 222bp downstream the TSS, on the RV strand of the gene.
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OmpA is a predominant outer membrane protein in <i>Enterobacteriaceae</i>. Its expression can be influenced by various environmental factors, among them the one exercised by glucose is the most discussed. In <i>Klebsiella pneumoniae</i>, KpOmpA mediates adhesion to a wide range of immune effector cells, facilitating respiratory tract and urinary infections. It stabilizes Gram-negative bacteria by anchoring their outer membrane to the peptidoglycan layer.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 14:11, 7 October 2023


sgDNA OmpA 222 for K. pneumoniae

This guide DNA has been designed to work with dCas9 to target AMR related genes in Klebsiella pneumoniae. The guide binds 222bp downstream the TSS, on the RV strand of the gene.

OmpA is a predominant outer membrane protein in Enterobacteriaceae. Its expression can be influenced by various environmental factors, among them the one exercised by glucose is the most discussed. In Klebsiella pneumoniae, KpOmpA mediates adhesion to a wide range of immune effector cells, facilitating respiratory tract and urinary infections. It stabilizes Gram-negative bacteria by anchoring their outer membrane to the peptidoglycan layer.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 17
  • 1000
    COMPATIBLE WITH RFC[1000]