Difference between revisions of "Part:BBa K200013:Design"

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===Design Notes===
 
===Design Notes===
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Since polypeptides can only be biologically synthesised if they start with the amino acid methionine, it has been necessary to design a cleavable peptide sequence starting with methionine. This sequence is found upstream of opiorphin and is enzymatically cleaved following incubation with the enzyme enterokinase. Enterokinase is a highly specific serine protease that cleaves after the recognition sequence Aspartic Acid - Aspartic Acid - Aspartic Acid -Aspartic Acid – Lysine.
 
  
 +
==Cleavable Linker==
  
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https://static.igem.org/mediawiki/2009/a/a2/Opiorphin.png
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Since polypeptides can only be biologically synthesised if they start with the amino acid methionine, it has been necessary to design a cleavable peptide sequence starting with methionine. This sequence is found upstream of opiorphin and is enzymatically cleaved following incubation with the enzyme enterokinase. Enterokinase is a highly specific serine protease that cleaves after the recognition sequence Aspartic Acid - Aspartic Acid - Aspartic Acid -Aspartic Acid – Lysine.  
  
−
The methionine is shown in RED, the protease recognition site is shown in BLUE and opiorphin is shown in GREEN. The RED & BLUE sections make up the removable linker region cleaved by the serine protease enterokinase.  
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[[Image:https://static.igem.org/mediawiki/2009/a/a2/Opiorphin.png]]
  
 +
The methionine is shown in RED, the protease recognition site is shown in BLUE and opiorphin is shown in GREEN. The RED & BLUE sections make up the removable linker region which is cleaved by the serine protease enterokinase.
  
 +
==Stop Codon==
  
 
A double stop codon has been incorporated into the sequence to ensure efficient termination.
 
A double stop codon has been incorporated into the sequence to ensure efficient termination.
 +
 +
 +
==Codon Choice==
  
 
The sequence has been optimised for expression in <i>E.coli</i>.
 
The sequence has been optimised for expression in <i>E.coli</i>.

Revision as of 15:35, 18 October 2009

Opiorphin + EK cleavage site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cleavable Linker

Since polypeptides can only be biologically synthesised if they start with the amino acid methionine, it has been necessary to design a cleavable peptide sequence starting with methionine. This sequence is found upstream of opiorphin and is enzymatically cleaved following incubation with the enzyme enterokinase. Enterokinase is a highly specific serine protease that cleaves after the recognition sequence Aspartic Acid - Aspartic Acid - Aspartic Acid -Aspartic Acid – Lysine.

File:Https://static.igem.org/mediawiki/2009/a/a2/Opiorphin.png

The methionine is shown in RED, the protease recognition site is shown in BLUE and opiorphin is shown in GREEN. The RED & BLUE sections make up the removable linker region which is cleaved by the serine protease enterokinase.

Stop Codon

A double stop codon has been incorporated into the sequence to ensure efficient termination.


Codon Choice

The sequence has been optimised for expression in E.coli.

Source

Synthetic part.

References