Difference between revisions of "Part:BBa K4897001"

Revision as of 13:53, 7 October 2023

BS DNA-162 (BS DNA 2.0) using in L-RCA for detecting P. acne

BS DNA-162 was designed by BS United China as a single-stranded DNA segment complementary to the 131 base pairs of the 16S rRNA gene of P. acne. The composition of the DNA has three categories: binding region (two ends), amplification region, and random region. The binding region is the key element in reacting with the P. acne 16s rRNA gene. The DNA ligase will perform the ligation of the single-stranded DNA meanwhile the phi29 will generate double-stranded DNA through amplification primers

Usage and Biology

BS DNA-162 would bind the bacterial DNA of P. acne and show great sensitivity and specificity.

Characterization

<img src="

" width="200" height="auto" />

Fig.1 Characterization

Lane 1 is a 100 bp DNA ladder. Lane 2 is a positive control tested by synthetic and isolated P. acne DNA. Lane 3 is a positive result tested by samples from human faces through washing.

The positive control and the positive result both have amplification results beyond 162, the initial size, suggesting successful DNA ligation and amplification.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 83
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 57
Illegal SpeI site found at 83
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 68
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 83
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 83
1000
COMPATIBLE WITH RFC[1000]

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-<img src="https://static.igem.wiki/teams/4897/wiki/parts/bs-dna-162.png" width="200" height="auto" />
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-Lane 1 is a 100 bp DNA ladder. Lane 2 is a positive control tested by synthetic and isolated P. acne DNA. Lane 3 is a positive result tested by samples from human faces through washing.
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+Lane 1 is a 100 bp DNA ladder. Lane 2 is a positive control tested by synthetic and isolated P. acne DNA. Lane 3 is a positive result tested by samples from human faces through washing.
 The positive control and the positive result both have amplification results beyond 162, the initial size, suggesting successful DNA ligation and amplification.