Difference between revisions of "Part:BBa K4395013"
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===Characterization from iGEM23-NJTech-China-B=== | ===Characterization from iGEM23-NJTech-China-B=== | ||
| + | ===YahK=== | ||
| + | YahK, an aldehyde reductase from Escherichia coli, catalyzes the reduction of various aldehydes to corresponding alcohols. YahK has been found to be important in NADP/NADPH homeostasis, lipid biosynthesis, amino acid metabolism, or the formation of fuel alcohols. The broad substrate range of YahK implies its more universal function. In our experiment, YahK was used to reduce Glutaraldehyde to produce pentanediol. | ||
Revision as of 13:21, 7 October 2023
NADPH-dependent aldehyde reductase YahK catalyzes the reduction of a wide range of aldehydes into their corresponding alcohols. Has a strong preference for NADPH over NADH as the electron donor. Cannot use a ketone as substrate. Is a major source of NADPH-dependent aldehyde reductase activity in E.coli[1]. In addition, this enzyme has been actively expressed in E.coli[2].These characteristics of RsAs attract us and thus we choose it as aldehyde reductase of our experiment.
Reference: 1.Pick A, Rühmann B, Schmid J, Sieber V. Novel CAD-like enzymes from Escherichia coli K-12 as additional tools in chemical production. Appl Microbiol Biotechnol. 2013;97(13):5815-5824. doi:10.1007/s00253-012-4474-5 2.Kramer L, Le X, Rodriguez M, Wilson MA, Guo J, Niu W. Engineering Carboxylic Acid Reductase (CAR) through a Whole-Cell Growth-Coupled NADPH Recycling Strategy. ACS Synth Biol. 2020;9(7):1632-1637. doi:10.1021/acssynbio.0c00290
Contribution From NJTech-China-B 2023
Group:iGEM NJTech-China-B
Author: Yao Yao
Characterization from iGEM23-NJTech-China-B
YahK
YahK, an aldehyde reductase from Escherichia coli, catalyzes the reduction of various aldehydes to corresponding alcohols. YahK has been found to be important in NADP/NADPH homeostasis, lipid biosynthesis, amino acid metabolism, or the formation of fuel alcohols. The broad substrate range of YahK implies its more universal function. In our experiment, YahK was used to reduce Glutaraldehyde to produce pentanediol.