Difference between revisions of "Part:BBa K200013"
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See 'Design Notes' for our rationale behind incorporating an EK cleavage sequence on the N terminus of the opiorphin pentapeptide. | See 'Design Notes' for our rationale behind incorporating an EK cleavage sequence on the N terminus of the opiorphin pentapeptide. | ||
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In an in-vitro bioassay it was shown that opiorphin (in concentarions 10<sup>−6</sup> to 10<sup>−4</sup> M) caused colonic contraction in a concentration-dependent manner <sup>2</sup>. Based on this evidence, opiorphin could have potential as an antidiarrheal agent. | In an in-vitro bioassay it was shown that opiorphin (in concentarions 10<sup>−6</sup> to 10<sup>−4</sup> M) caused colonic contraction in a concentration-dependent manner <sup>2</sup>. Based on this evidence, opiorphin could have potential as an antidiarrheal agent. | ||
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Revision as of 15:03, 18 October 2009
Opiorphin + EK cleavage site
Usage and Biology
Background
Opiorphin is a pentapeptide (five amino acids long) that was isolated from human saliva in 1996. Opiorphin acts as a pain killing agent. The amino acid sequence for opiorphin is Glutamine-Arginine-Phenylalanine-Serine-Arginine.
Mechanism of Action
Preliminary rat studies indicate that opiorphin has a pain killing effect six times stronger than that of morphine. With regards to its mechanism of action, opiorphin prevents the breakdown of enkephalins which are natural pain-killing opioids.
EK Cleavage
See 'Design Notes' for our rationale behind incorporating an EK cleavage sequence on the N terminus of the opiorphin pentapeptide.
Side Effects
Priapism:
A recent study involved genetically engineering retired breeder rats to constitutively express opiorphin. Interestingly, this resulted in the development of a priapic-like condition. It is thought that this condition resulted from the upregulation of the ornithine decarboxylase gene (ODC) by opiorphin 1.
Antidiarrheal:
In an in-vitro bioassay it was shown that opiorphin (in concentarions 10−6 to 10−4 M) caused colonic contraction in a concentration-dependent manner 2. Based on this evidence, opiorphin could have potential as an antidiarrheal agent.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
<biblio>
- 1 pmid=19657052
- 2 pmid=19442408
</biblio>