Difference between revisions of "Part:BBa K4604031"

Revision as of 09:31, 7 October 2023

K12 AdoCbl riboswitch This Biobrick is a riboswitch for AdoCbl, a bioavailable form of B12. Upon binding to AdoCbl, a conformational change happens within the riboswitch, negatively regulating the gene downstream of it. To verify the performance of the riboswitch we created several sensors with a fluorescent readout: BBa_K4604026, BBa_K4604027, BBa_K4604028 and BBa_K4604029.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 162
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K4604031 short</partinfo>
+This Biobrick is a riboswitch for AdoCbl, a bioavailable form of B12. Upon binding to AdoCbl, a conformational change happens within the riboswitch, negatively regulating the gene downstream of it. To verify the performance of the riboswitch we created several sensors with a fluorescent readout: BBa_K4604026, BBa_K4604027, BBa_K4604028 and BBa_K4604029.
-Riboswitches occur naturally as regulators of gene expression. Riboswitches are  encoded as DNA sequences which exert their regulatory effect upon transcription by folding into a complex 3D structure. Generally, a riboswitch can fold in such a way that the ribosome binding site (RBS) is available or unavailable for the ribosome. Upon binding to the target compound, like AdoCbl, a configurational change in the riboswitch changes the availability of the RBS. In E. coli  a riboswitch regulates the expression of the btuB gene which encodes for a corrine transporter protein BtuB. It has been shown that when sufficient AdoCbl is present, the riboswitch undergoes a conformational change resulting in lower btuB expression. To verify the performance of the riboswitch we created several sensors with a fluorescent readout: BBa_K4604026, BBa_K4604027, BBa_K4604028 and BBa_K4604029.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+Riboswitches occur naturally as regulators of gene expression. Riboswitches are  encoded as DNA sequences which exert their regulatory effect upon transcription by folding into a complex 3D structure. Generally, a riboswitch can fold in such a way that the ribosome binding site (RBS) is available or unavailable for the ribosome. Upon binding to the target compound, like AdoCbl, a configurational change in the riboswitch changes the availability of the RBS. In E. coli  a riboswitch regulates the expression of the btuB gene which encodes for a corrine transporter protein BtuB. It has been shown that when sufficient AdoCbl is present, the riboswitch undergoes a conformational change resulting in lower btuB expression.
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