Difference between revisions of "Part:BBa K2706008"

 
Line 7: Line 7:
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
 +
= Contribution:CUG-China 2023 =
 +
Microbial electron exchange with extracellular substrates,such as electrodes in BESs, is often called microbialextracellular electron transfer (EET). The most wel lcharacterized microbial EET pathway is the Mtr pathway of <i>S. oneidensis</i> MR-1. S. oneidensis MR-1 transfers electrons from the quinone/quinol pool in the cytoplasmic membrane to the bacterial surface through redox and structural proteins,including the inner membrane c-Cyt CymA, the periplasmicc-Cyts Fcc3 and STC, and the outer membrane porin-c-Cytcomplex MtrCAB. Eventually, electrons are transferred from the bacterial surface to extracellular acceptors directly via the outer membrane c-Cyts or via nanowires, or indirectly via self-secreted electron mediator flavins. <i>Shewanella</i> nanowires are the extensions of the outer membrane and periplasm in which the cell surface c-Cyts MtrC and OmcA are the key players for long-distance electron transfer.
 +
 +
https://static.igem.wiki/teams/4767/wiki/part/bba-k2706008.png
 +
 +
Fig. Extracellular electron transfer (EET) pathways of <i>Shewanella oneidensis</i> MR-1 to electrodes.
 +
 +
[1] Yidan Hu, Wang Yinghui, Han Xi, et al. Biofilm Biology and Engineering of <i>Geobacter</i> and <i>Shewanella</i> spp. for Energy Applications[J]. Frontiers in Bioengineering and Biotechnology, 2021, 9.
 +
[2] Liang Shi, Dong Hailiang, Reguera Gemma, et al. Extracellular electron transfer mechanisms between microorganisms and minerals[J]. Nature Reviews Microbiology, 2016, 14(10): 651-662.
  
 
<!-- -->
 
<!-- -->

Revision as of 09:27, 7 October 2023


cymA

Endogenous to Shewanella Onedensis, cymA can accept electrons in interior of cell membrane from MtrCAB complex

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 306
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]