Difference between revisions of "Part:BBa K2706008"
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | = Contribution:CUG-China 2023 = | ||
+ | Microbial electron exchange with extracellular substrates,such as electrodes in BESs, is often called microbialextracellular electron transfer (EET). The most wel lcharacterized microbial EET pathway is the Mtr pathway of <i>S. oneidensis</i> MR-1. S. oneidensis MR-1 transfers electrons from the quinone/quinol pool in the cytoplasmic membrane to the bacterial surface through redox and structural proteins,including the inner membrane c-Cyt CymA, the periplasmicc-Cyts Fcc3 and STC, and the outer membrane porin-c-Cytcomplex MtrCAB. Eventually, electrons are transferred from the bacterial surface to extracellular acceptors directly via the outer membrane c-Cyts or via nanowires, or indirectly via self-secreted electron mediator flavins. <i>Shewanella</i> nanowires are the extensions of the outer membrane and periplasm in which the cell surface c-Cyts MtrC and OmcA are the key players for long-distance electron transfer. | ||
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+ | https://static.igem.wiki/teams/4767/wiki/part/bba-k2706008.png | ||
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+ | Fig. Extracellular electron transfer (EET) pathways of <i>Shewanella oneidensis</i> MR-1 to electrodes. | ||
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+ | [1] Yidan Hu, Wang Yinghui, Han Xi, et al. Biofilm Biology and Engineering of <i>Geobacter</i> and <i>Shewanella</i> spp. for Energy Applications[J]. Frontiers in Bioengineering and Biotechnology, 2021, 9. | ||
+ | [2] Liang Shi, Dong Hailiang, Reguera Gemma, et al. Extracellular electron transfer mechanisms between microorganisms and minerals[J]. Nature Reviews Microbiology, 2016, 14(10): 651-662. | ||
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Revision as of 09:27, 7 October 2023
cymA
Endogenous to Shewanella Onedensis, cymA can accept electrons in interior of cell membrane from MtrCAB complex
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 306
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]