Difference between revisions of "Part:BBa K4767008"
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Xiaoqiang Jia, Bu Rongrong, Zhao Tingting, et al. Sensitive and Specific Whole-Cell Biosensor for Arsenic Detection[J]. Applied and environmental microbiology, 2019, 85(11): 1. | Xiaoqiang Jia, Bu Rongrong, Zhao Tingting, et al. Sensitive and Specific Whole-Cell Biosensor for Arsenic Detection[J]. Applied and environmental microbiology, 2019, 85(11): 1. | ||
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Nistala, G.J., et al., A modular positive feedback-based gene amplifier. J Biol Eng, 2010. 4: p. 4. | Nistala, G.J., et al., A modular positive feedback-based gene amplifier. J Biol Eng, 2010. 4: p. 4. | ||
Revision as of 06:48, 7 October 2023
Pars-RBS-arsR-RBS-luxR(△2-162)-TT
Uses a factor Pars(BBa_K4767001) ,strong RBS(BBa_J34801), DNA binding transcriptional repressor arsR(BBa_J15101), luxR(△2-162)(BBa_K4767000) and TT(BBa_B015).
Usage and Biology
After the modification of the LuxR(Δ2-162), we applied the amplifier into the arsenic-response system we build. The transcriptional activator, LuxR(Δ2-162) was regulated by the arsR-Pars circuit, while reporter gene together with a second LuxR(Δ2-162) was placed under the promoter PluxI, which was activated by LuxR(Δ2-162). When arsenic is present, it activates the expression of LuxR(Δ2-162) in the first circuit, which turns on the expression of reporter and LuxR(Δ2-162) (Part 5)from the following circuit.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 270
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Xiaoqiang Jia, Bu Rongrong, Zhao Tingting, et al. Sensitive and Specific Whole-Cell Biosensor for Arsenic Detection[J]. Applied and environmental microbiology, 2019, 85(11): 1.
Nistala, G.J., et al., A modular positive feedback-based gene amplifier. J Biol Eng, 2010. 4: p. 4.