Difference between revisions of "Part:BBa K196014:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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As many mutations were needed to make the part compatible with the standard 10, we decided to make hfsG and hfsH synthetized by GeneArt [[http://genearts.com/ 2]]. We also optimized it for E. coli. We mean that the codons were changed to favour the most present in E. coli.
+
As many mutations were needed to make the part compatible with the standard 10, we decided to make hfsG and hfsH synthetized by [http://genearts.com/ GeneArt]. We also optimized it for E. coli. We mean that the codons were changed to favour the most present in E. coli.
  
 
===Source===
 
===Source===

Latest revision as of 13:29, 18 October 2009

Final GluColi device.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2263
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1273
    Illegal AgeI site found at 636
    Illegal AgeI site found at 748
    Illegal AgeI site found at 1172
    Illegal AgeI site found at 1657
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As many mutations were needed to make the part compatible with the standard 10, we decided to make hfsG and hfsH synthetized by [http://genearts.com/ GeneArt]. We also optimized it for E. coli. We mean that the codons were changed to favour the most present in E. coli.

Source

HfsG and HfsH sequences come from Caulobacter crescentus.

References