Difference between revisions of "Part:BBa K4767006"
| Line 3: | Line 3: | ||
<partinfo>BBa_K4767006 short</partinfo> | <partinfo>BBa_K4767006 short</partinfo> | ||
| − | Uses a factor Pars(BBa_K4767001) ,strong RBS(BBa_J34801), DNA binding transcriptional repressor arsR(BBa_J15101), cymA(BBa_K2706008) and TT( | + | Uses a factor Pars(BBa_K4767001) ,strong RBS(BBa_J34801), DNA binding transcriptional repressor <i>arsR</i>(BBa_J15101), c-type cytochromes <i>cymA</i>(BBa_K2706008) and double terminator TT(BBa_B015). This part is an easy BioBrick. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | This part is a arsenic reporter with the reporter gene <i>cymA</i>. CymA is a c-type cytochromes from <i>Shewanella oneidensis</i>. It plays an important role in its extracellular electron transfer. To verify this part can respond to arsenic, we transfer this part into a <i>S. oneidensis</i> strain with the <i>cymA</i> gene mutant. Then we cultured the strain with a arsenic concentration gradient. When arsenic is absent, the transcription regulator ArsR binds to the ArsR-binding site (ABS) within the <i>ars</i> promoter and blocks transcription. Once arsenic is present, it binds to ArsR and activate the transcription of the <i>cymA</i> genes. As the result showed, strains with higher arsenic has a faster iron reduction rate, indicating the more expression of <i>cymA</i>. | ||
| + | |||
| + | [[File:BBa_K4767006.jpg]] | ||
| + | |||
| + | Fig. Iron reduction curves with different arsenic concentrations. | ||
<!-- --> | <!-- --> | ||
| Line 12: | Line 17: | ||
<partinfo>BBa_K4767006 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4767006 SequenceAndFeatures</partinfo> | ||
| + | ===References=== | ||
| + | Xiaoqiang Jia, Bu Rongrong, Zhao Tingting, et al. Sensitive and Specific Whole-Cell Biosensor for Arsenic Detection[J]. Applied and environmental microbiology, 2019, 85(11): 1. | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
Revision as of 06:11, 7 October 2023
Pars-RBS-arsR-RBS-cymA-TT
Uses a factor Pars(BBa_K4767001) ,strong RBS(BBa_J34801), DNA binding transcriptional repressor arsR(BBa_J15101), c-type cytochromes cymA(BBa_K2706008) and double terminator TT(BBa_B015). This part is an easy BioBrick.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 270
Illegal BglII site found at 824 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Xiaoqiang Jia, Bu Rongrong, Zhao Tingting, et al. Sensitive and Specific Whole-Cell Biosensor for Arsenic Detection[J]. Applied and environmental microbiology, 2019, 85(11): 1.