Difference between revisions of "Part:BBa K4767005"

Revision as of 06:01, 7 October 2023

PluxⅠ-RBS-luxR(△2-162)-RBS-gfp-TT

Uses a factor PluxⅠ(BBa_R0062) ,strong RBS(BBa_J34801), luxR(△2-162)(BBa_K4767000) and gfp coding region(BBa_E0040)and TT(BBa_B015). This part is an easy BioBrick.

Usage and Biology

This is the response part of the luxR amplifier with the gfp gene as the reporter. In our design, if luxR(△2-162) was expressed as a result of responding, this part can be activated to express gfp and luxR(△2-162), and luxR(△2-162) will functions in a positive feedback loop as it can bind to the PluxI promoter and activate its own transcription.

File:BBa K4767005.jpg

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1021

References

Xiaoqiang Jia, Bu Rongrong, Zhao Tingting, et al. Sensitive and Specific Whole-Cell Biosensor for Arsenic Detection[J]. Applied and environmental microbiology, 2019, 85(11): 1.

@@ Line 9: / Line 9: @@
 This is the response part of the <i>luxR</i> amplifier with the <i>gfp</i> gene as the reporter. In our design, if <i>luxR</i>(△2-162) was expressed as a result of responding, this part can be activated to express <i>gfp</i> and <i>luxR</i>(△2-162), and <i>luxR</i>(△2-162) will functions in a positive feedback loop as it can bind to the PluxI promoter and activate its own transcription.
-[[File:BBa_K4767005.jpg]]
+<center>[[File:BBa_K4767005.jpg]]</center>
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