Difference between revisions of "Part:BBa K4767004"
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===Usage and Biology===
 
===Usage and Biology===
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This part is a basic biosensor for arsenic detection using <i>gfp</i> as the reporter gene. When arsenic is absent, the transcription regulator ArsR binds to the ArsR-binding site (ABS) within the <i>ars</i> promoter and blocks transcription. Once arsenic is present, it binds to ArsR and activate the transcription of the downstream genes. Since this <i>ars</i>
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System comes from <i>Escherichia coli</i> genome, to eliminate this impact, we used another model bacteria <i>Shewanella oneidensis</i> as the chassis cells to verify the function of this part. As the result shows below, with the arsenic concentration rises, the strain with the reporter produced higher fluorescence intensity.
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[[File:BBa_K4767004.jpg]]
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Fig. Fluorescence curve of the reporter strain with different arsenic concentrations
  
 
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Revision as of 03:25, 7 October 2023


Pars-RBS-arsR-RBS-gfP-TT

Uses a factor Pars(BBa_K4767001) ,strong RBS(BBa_J34801), DNA binding transcriptional repressor arsR(BBa_J15101), gfp coding region(BBa_E0040)and double terminator TT(BBa_B015). This part is an easy BioBrick.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 270
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1160