Difference between revisions of "Part:BBa K173004"
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{|align="center"
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|[[Image:.pv_BBa K173004.png|center|thumb|200px|Fig.1: lactose cleavage to glucose and galactose.]]
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|[[Image:.pv_BBa K173004.png|center|thumb|220px|Fig.1: lactose cleavage to glucose and galactose.]]
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|[[Image:.pv_BBa K173004_xg.png|center|thumb|350px|Fig.2: X-Gal cleavage to galactose and an insoluble blue product.]]
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|[[Image:.pv_BBa K173004_xg.png|center|thumb|380px|Fig.2: X-Gal cleavage to galactose and an insoluble blue product.]]
 
|[[Image:.pv_BBa K173004_colonies.png|center|thumb|100px|Fig.3: example of blue colonies bearing lacZ.]]
 
|[[Image:.pv_BBa K173004_colonies.png|center|thumb|100px|Fig.3: example of blue colonies bearing lacZ.]]
 
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Revision as of 12:16, 18 October 2009

Beta-galactosidase protein generator

Beta-galactosidase protein generator with strong RBS.

This part takes PoPS as input to express lacZ gene (BBa_I732005), encoding for beta-galactosidase enzyme. This enzyme can be used to cleave lactose molecule to glucose and galactose (see figure), but can also be used as a reporter protein for colorimetric assays (together with X-Gal or ONPG as a substrate).

Fig.1: lactose cleavage to glucose and galactose.
Fig.2: X-Gal cleavage to galactose and an insoluble blue product.
Fig.3: example of blue colonies bearing lacZ.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]