Difference between revisions of "Part:BBa K173004"

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|[[Image:.pv_BBa K173004.png|center|200px|caption="a"]]
 
|[[Image:.pv_BBa K173004_xg.png|center|350px]]
 
|[[Image:.pv_BBa K173004_xg.png|center|350px]]
 
|[[Image:.pv_BBa K173004_colonies.png|center|200px]]
 
|[[Image:.pv_BBa K173004_colonies.png|center|200px]]

Revision as of 12:10, 18 October 2009

Beta-galactosidase protein generator

Beta-galactosidase protein generator with strong RBS.

This part takes PoPS as input to express lacZ gene (BBa_I732005), encoding for beta-galactosidase enzyme. This enzyme can be used to cleave lactose molecule to glucose and galactose (see figure), but can also be used as a reporter protein for colorimetric assays (together with X-Gal or ONPG as a substrate).

caption="a"
.pv BBa K173004 xg.png
.pv BBa K173004 colonies.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]