Difference between revisions of "Part:BBa K4634011"
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+ | ===References=== | ||
+ | [1] Movva NR, Nakamura K, Inouye M. Amino acid sequence of the signal peptide of ompA protein, a major outer membrane protein of Escherichia coli. J Biol Chem. 1980 Jan 10;255(1):27-9. PMID: 6985608. |
Latest revision as of 17:52, 6 October 2023
OmpA-mCherry
The OmpA signal peptide(BBa_K4380001) is a short amino acid sequence found at the N-terminus of the OmpA protein in Gram-negative bacteria. The signal peptide acts as a recognition signal for the protein translocation machinery present in the bacterial cell. In the composite part OmpA-mCherry, 21aa long OmpA signal peptide is linked at the N terminal of mCherry(BBa_K2244008), which is used to test the function of OmpA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 748
- 1000COMPATIBLE WITH RFC[1000]
References
[1] Movva NR, Nakamura K, Inouye M. Amino acid sequence of the signal peptide of ompA protein, a major outer membrane protein of Escherichia coli. J Biol Chem. 1980 Jan 10;255(1):27-9. PMID: 6985608.