Difference between revisions of "Part:BBa K4604015:Design"

 
Line 8: Line 8:
 
===Design Notes===
 
===Design Notes===
 
The start codon of the bluB was changed to ATG to act as a start for translation in E. coli.
 
The start codon of the bluB was changed to ATG to act as a start for translation in E. coli.
−
Correct overhangs had to be designed to ensure high efficiency with Golden Gate.  
+
The region downstream of the tetA/B promoter was shortened (in comparison to BBa_K4604016) to counteract the leakiness.  
  
  
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===Source===
 
===Source===
  
−
Assembled with Golden Gate cloning.
+
Modified piG_01a using Gibson Assembly.
  
 
===References===
 
===References===

Revision as of 16:31, 6 October 2023


piG_01b (tetR_bluB)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 710
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1618


Design Notes

The start codon of the bluB was changed to ATG to act as a start for translation in E. coli. The region downstream of the tetA/B promoter was shortened (in comparison to BBa_K4604016) to counteract the leakiness.



Source

Modified piG_01a using Gibson Assembly.

References