Difference between revisions of "Part:BBa K4604015"
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===Usage and Biology===
 
===Usage and Biology===
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This BioBrick can be used to produce Adenosylcobalamin (vitamin B12) in E. coli when supplemented with cobinamide.
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The tet promoter used in this version of the plasmid is a modified version of BBa_K4604016, piG_01a.
  
 
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Revision as of 16:28, 6 October 2023


piG_01b (tetR_bluB)

piG_01b is a plasmid consisting of the tet promoter/repressor, a functional bluB gene and the rrnB terminator. The backbone we used in the experiments is pGGAselect.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 710
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1618