Difference between revisions of "Part:BBa K4604011"
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<partinfo>BBa_K4604011 short</partinfo> | <partinfo>BBa_K4604011 short</partinfo> | ||
| − | MazF | + | In this BioBrick we fused MazF together with a FLAG-tag using a 3X Alanine linker. This FLAG-tag can be used for protein detection in western blot. Used in Biobricks BBa_K4604017, BBa_K4604018, BBa_K4604019, BBa_K4604021, BBa_K4604022, BBa_K4604023, BBa_K4604024 and BBa_K4604025. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | MazF is a protein that acts as an endoribonuclease. It forms a dimer and can be neutralized by mazE, an antitoxin. | ||
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Revision as of 16:08, 6 October 2023
MazF toxin
In this BioBrick we fused MazF together with a FLAG-tag using a 3X Alanine linker. This FLAG-tag can be used for protein detection in western blot. Used in Biobricks BBa_K4604017, BBa_K4604018, BBa_K4604019, BBa_K4604021, BBa_K4604022, BBa_K4604023, BBa_K4604024 and BBa_K4604025.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]