Difference between revisions of "Part:BBa K233006:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | We obtained a lac operator and paired it with phage T7 promoter. Hence we have an AND gate which requires both the inputs- T7 polymerase and IPTG/lactose in order for the gene downstream to be transcribed. | |
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===Source=== | ===Source=== | ||
Latest revision as of 11:44, 18 October 2009
AND Gate 3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We obtained a lac operator and paired it with phage T7 promoter. Hence we have an AND gate which requires both the inputs- T7 polymerase and IPTG/lactose in order for the gene downstream to be transcribed.
Source
E.coli phage t7