Difference between revisions of "Part:BBa K233005:Design"

 
(Design Notes)
 
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<partinfo>BBa_K233005 short</partinfo>
 
<partinfo>BBa_K233005 short</partinfo>
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===Design Notes===
 
===Design Notes===
synthesized lacO and added it to the pLL biobrick
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We obtained a lac operator and paired it with phage pLL promoter. Hence we have an AND gate which requires both the inputs- phage encoded protein Ogr and IPTG/lactose in order for the gene downstream to be transcribed.
 
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===Source===
 
===Source===

Latest revision as of 11:41, 18 October 2009

AND gate 2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We obtained a lac operator and paired it with phage pLL promoter. Hence we have an AND gate which requires both the inputs- phage encoded protein Ogr and IPTG/lactose in order for the gene downstream to be transcribed.

Source

E.coli- lac operator pLL- phage p4

References