Difference between revisions of "Part:BBa K233003:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
 
we had a lac binding site without any promoter activity synthesized.
 
we had a lac binding site without any promoter activity synthesized.
−
We obtained a lac operator and paired it with pLuxR. Hence we have an AND gate with
+
We obtained a lac operator and paired it with pLuxR. Hence we have an AND gate which requires both the inputs- LuxR/AHL and IPTG/lactose in order for the gene downstream to be transcribed
  
 
===Source===
 
===Source===

Revision as of 11:35, 18 October 2009

AND Gate 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

we had a lac binding site without any promoter activity synthesized. We obtained a lac operator and paired it with pLuxR. Hence we have an AND gate which requires both the inputs- LuxR/AHL and IPTG/lactose in order for the gene downstream to be transcribed

Source

Promoter biobricks Lac operator- E.coli

References