Difference between revisions of "Part:BBa K4897010"

Revision as of 14:02, 6 October 2023

TurboID-FGB

TurboID was fused with human beta-fibrinogen which can target the P. acne's surface protein. The interaction of human beta-fibrinogen with P. acne surface protein will lead to the proximity of the Biotinylation of P. acne surface proteins. Thus, the biotinylation of surface proteins will block the signaling and quorum sensing. 

P. acne can attach to human skin and cause infection by traveling from the surface to its infection site. When it reach the infection site and bind to human epithelial cells, this process is irreversible and triggers a series of reaction, initiating bacterial colonization, production of reactive oxygen species, and inflammatory reaction. Thus, preventing P. acne from binding human cells is a crucial mission of BS_United China_2023 to suppress P. acne’s virulence. Specifically, P. acne binds to a blood plasma protein called fibrinogen (FBG). The surface of P. acne contains largely a certain protein, in size of 58 kDa which binds strongly to the N-terminal Fg1 peptide, one of the components of beta-fibrinogen. Thus, P. acne strongly binds to human fibrinogen through its surface protein which we call fibrinogen binding protein. 

Modifying our last year’s project, TurboID, we successfully adopted it to target the P. acne surface protein, the fibrinogen binding protein. FGB would direct TurboID to bind a large number of biotin onto the surface proteins of P. acne when FGB binds to the fibrinogen-binding protein of P. acne.

As FGB directs TurboID toward the fibrinogen binding protein of P. acne, TurboID will catalyze biotin and ATP it carries to produce Biotinyl 5’-AMP for protein labeling. Within a maximum range of 10 nanometers, TurboID is able to label P. acne’s surface proteins with Biotinyl5’-AMP. Moreover, by using streptavidin-phycoerythrin which binds strongly to biotin, we can cover a large surface area when letting it form strong bonds with biotin. As a result, P. acne’s communication with the outside world is completely cut off: the metabolic waste cannot be exported out of the cell, and nutrients cannot be transported into the cell. P. acne is suppressed by TurboID-FGB.

Sequence and Features