Difference between revisions of "Part:BBa K4677003"

Revision as of 13:25, 6 October 2023

TorT-TorS

The TMAO reductase pathway is controlled by a receptor system that comprises the TMAO-binding protein TorT, the sensor histidine kinase TorS, and the response regulator TorR. TorT associates as a coreceptor with the histidine kinase receptor TorS to form the sensor complex. TorT-TorS is an asymmetric 2:2 complex that binds TMAO with negative cooperativity to form a symmetric active kinase. TMAO-stimulated TorS phosphorylates the response regulator TorR; phosphorylated TorR activates transcription from the torCAD operon to express TorCAD which are involved in the TMAO reductase pathway.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1361
Illegal XhoI site found at 3615
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 42
Illegal AgeI site found at 3366
1000
COMPATIBLE WITH RFC[1000]

In order to construct the standard part pSB1C3-TorT-TorS plasmid, TorT-TorS sequence was tested to see if there is EcoRI and PstI site. The testing result was showed in Fig.1.

File:K4677003-1.jpg

Fig.1 The map of TorT-TorS sequence described by SnapGene Viewer, showing the restriction enzyme information (no EcoRI and PstI sites).

File:K4677003-2.jpg

File:K4677003-3.jpg

@@ Line 17: / Line 17: @@
 <partinfo>BBa_K4677003 parameters</partinfo>
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+<br/>
+<br/>
+In order to construct the standard part pSB1C3-TorT-TorS plasmid, TorT-TorS sequence was tested to see if there is EcoRI and PstI site. The testing result was showed in Fig.1.
+<br/>
+[[File:K4677003-1.jpg|center]]
+Fig.1 The map of TorT-TorS sequence described by SnapGene Viewer, showing the restriction enzyme information (no EcoRI and PstI sites).
+<br/>
+[[File:K4677003-2.jpg|center]]
+<br/>
+[[File:K4677003-3.jpg|center]]