Difference between revisions of "Part:BBa K4890015"
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   < img class="bild" src="https://static.igem.wiki/teams/4890/wiki/parts/14-01-15-01-gel-electrophoresis-of-mre.png">
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   <div class="unterschrift"><b>Fig.1 Construct design</b>
 
   <div class="unterschrift"><b>Fig.1 Construct design</b>
 
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Revision as of 11:30, 6 October 2023


MRE-Hsp70-GFP This part is responsible to the expression of GFP gene driven by MRE in Drosophila. It consists of MRE sequence (BBa_K4890002), Hsp70 sequence (BBa_K4890004) and GFP gene (BBa_S03452). Metal response element (MRE) is derived from Drosophila melanogaster. In the cell nucleus, activated MTF-1 binds to MRE. MRE is typically located in the promoter regions associated with genes involved in the response to heavy metals. Binding of MTF-1 to MRE activates the transcription of the corresponding genes, thereby promoting heavy metal-related gene expression. GFP gene is derived from plasmid pcDNA3.1-CMV.GFP which was kept by Genetic Lab, School of Life science and Technology, Tongji University. It encodes green fluorescent protein (GFP) that emits green fluorescence when exposed to light in the blue to ultraviolet range. Hsp70 is derived from pUAST plasmid. Hsp70 is a promoter that can bind to RNA polymerase and start transcription. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 196
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 196
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 196
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 196
  • 1000
    COMPATIBLE WITH RFC[1000]


< img class="bild" src="https://static.igem.wiki/teams/4890/wiki/parts/1006.jpg">

Fig.1 Construct design