Difference between revisions of "Part:BBa K4677001"
Line 19: Line 19:
 
<br/>
 
<br/>
 
<br/>
 
<br/>
−
This part contains the CcpAx protein, also known as ORF-2, encoded by ccpax gene, functions as a mediator of protein-protein interactions and is important for localization of the bacterial cellulose synthase complex to the cell membrane in Gluconacetobacter xylinus. CcpAx gene, containing 936bp, was amplified using PCR method, and then inserted into the vector pSB1C3. The identification result is showed in Fig.1.
+
In order to construct the standard part pSB1C3-TorR plasmid, TorR sequence was tested to see if there is EcoRI and PstI site. The testing result was showed in Fig.1.  
 
<br/>
 
<br/>
−
[[File:K3605005-1.jpg|center]]
+
[[File:K4677001-1.jpg|center]]
−
Fig.1. The result of CcpAx gene cloning.
+
Fig.1 The map of TorR gene sequence described by SnapGene Viewer, showing the restriction enzyme information (no EcoRI and PstI sites).
−
M: Marker; 1: PCR result of CcpAx; 2: Digestion of pSB1C3 containing CcpAx.
+

Revision as of 10:54, 6 October 2023


TorR

The torR gene of Escherichia coli encodes a 25kD response regulator protein involved in the expression regulation of the trimethylamine N-oxide reductase genes, which are regulated by torCAD operon. The torR gene is located just upstream of the torCAD operon, with an opposite transcription direction. The torR-torCAD intergenic region is unusual in that it contains four direct repeats of a 10-nucleotide motif. Part or all of these motifs could be involved in the binding of TorR protein. TorR only mediate the TMAO induction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]




In order to construct the standard part pSB1C3-TorR plasmid, TorR sequence was tested to see if there is EcoRI and PstI site. The testing result was showed in Fig.1.

File:K4677001-1.jpg

Fig.1 The map of TorR gene sequence described by SnapGene Viewer, showing the restriction enzyme information (no EcoRI and PstI sites).