Difference between revisions of "Part:BBa K4724000:Design"

(→‎References)
(→‎References)
Line 16: Line 16:
  
 
===References===
 
===References===
−
[1]Hwang, D.-H. et al. Enhanced biodegradation of waste poly(ethylene terephthalate) using a reinforced plastic degrading enzyme complex. Science of The Total Environment 842, 156890 (2022).
+
[1] Hwang, D.-H. et al. Enhanced biodegradation of waste poly(ethylene terephthalate) using a reinforced plastic degrading enzyme complex. Science of The Total Environment 842, 156890 (2022).
  
−
[2]Zhang, Y. et al. Interactions between type A carbohydrate binding modules and cellulose studied with a quartz crystal microbalance with dissipation monitoring. Cellulose 27, 3661–3675 (2020)
+
[2] Zhang, Y. et al. Interactions between type A carbohydrate binding modules and cellulose studied with a quartz crystal microbalance with dissipation monitoring. Cellulose 27, 3661–3675 (2020)

Revision as of 07:02, 6 October 2023


CBM3-6xHis Tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 70
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 70
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 448
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 70
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 70
    Illegal NgoMIV site found at 28
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

You must use a flexible amino acid chain long enough to link it with protein.


Source

From Bacillus subtilis

References

[1] Hwang, D.-H. et al. Enhanced biodegradation of waste poly(ethylene terephthalate) using a reinforced plastic degrading enzyme complex. Science of The Total Environment 842, 156890 (2022).

[2] Zhang, Y. et al. Interactions between type A carbohydrate binding modules and cellulose studied with a quartz crystal microbalance with dissipation monitoring. Cellulose 27, 3661–3675 (2020)