Difference between revisions of "Part:BBa K4579033"

Revision as of 05:00, 6 October 2023

pBTK1028 assembly backbone

Following the part type standard of the Yeast and Bee Microbiome Toolkits, pBTK1028 is a type 56781 BsaI dropout vector that can be used to create first stage assemblies by combining with type 2, 3, and 4 parts according to the BTK/YTK standard. It can also be used as a BsmBI dropout vector for second stage assembly of parts begining with the ConLS overhang and ending with the ConRE overhang. This backbone was created by Shaunak Kar from the Ellington lab at The University of Texas at Austin.

The first four bases of this part correspond to a type 5 part forward prefix, and the last four bases of this part correspond to a type 1 part reverse suffix.

For literature describing the BTK and YTK standards, please see the References section for this part.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2237
Illegal XbaI site found at 1229
Illegal XbaI site found at 2246
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2237
Illegal NheI site found at 2071
Illegal NotI site found at 182
Illegal NotI site found at 2225
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2237
Illegal BglII site found at 1070
23
INCOMPATIBLE WITH RFC[23]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2237
Illegal XbaI site found at 1229
Illegal XbaI site found at 2246
25
INCOMPATIBLE WITH RFC[25]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2237
Illegal XbaI site found at 1229
Illegal XbaI site found at 2246
Illegal NgoMIV site found at 105
Illegal NgoMIV site found at 406
Illegal AgeI site found at 1661
Illegal AgeI site found at 1985
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SapI site found at 346
Illegal SapI site found at 556

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4579033 short</partinfo>
-Following the part type standard of the Bee Toolkit, pBTK1028 is a type 56781 entry vector part that can be used to create assemblies by combining with type 2, 3, and 4 parts according to the BTK standard. This backbone was created for the BTK by Shaunak Kar, with permission for the UT iGEM team to use it as a basis for our composite part assemblies.
+Following the part type standard of the Yeast and Bee Microbiome Toolkits, pBTK1028 is a type 56781 BsaI dropout vector that can be used to create first stage assemblies by combining with type 2, 3, and 4 parts according to the BTK/YTK standard. It can also be used as a BsmBI dropout vector for second stage assembly of parts begining with the ConLS overhang and ending with the ConRE overhang. This backbone was created by Shaunak Kar from the Ellington lab at The University of Texas at Austin.
 The first four bases of this part correspond to a type 5 part forward prefix, and the last four bases of this part correspond to a type 1 part reverse suffix. https://static.igem.wiki/teams/4579/wiki/btk-overhangs-low-ish-res.png
-For additional info on the BTK standard, see: Leonard, S. P., Perutka, J., Powell, J. E., Geng, P., Richhart, D. D., Byrom, M., ... & Barrick, J. E. (2018). Genetic engineering of bee gut microbiome bacteria with a toolkit for modular assembly of broad-host-range plasmids. <i>ACS Synthetic Biology, 7</i>(5), 1279-1290.
+For literature describing the BTK and YTK standards, please see the References section for this part.