Difference between revisions of "Part:BBa K3880007"

Revision as of 04:26, 6 October 2023

pro24800 promoter

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Usage, Biology and Characterization

SCUT-China 2023's Characterization

Pro1007(P24880) contains the promoter of GQS52_24880 in SCUT-3. The fusion promoter composed of Pro1007（BBa_K3880007）and ProP（BBa_K4621004）can control the gene expression switch while controlling its expression intensity.

Pro1007 was inserted into the chitin-inducible promoter ProP to form a fusion promoter. The regulation of gene expression can be achieved by placing it before the coding of the target gene. In our study, Pro1007 stably expressed the target gene under the premise of chitin induction to control the production of ectoine and hydroxyectoine. We verified its regulation effect by LB fermentation.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 120
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 162
1000
COMPATIBLE WITH RFC[1000]

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+===Usage, Biology and Characterization===
+== SCUT-China 2023's Characterization ==
+Pro1007(P24880) contains the promoter of GQS52_24880 in SCUT-3. The fusion promoter composed of Pro1007（BBa_K3880007）and ProP（BBa_K4621004）can control the gene expression switch while controlling its expression intensity.
+Pro1007 was inserted into the chitin-inducible promoter ProP to form a fusion promoter. The regulation of gene expression can be achieved by placing it before the coding of the target gene. In our study, Pro1007 stably expressed the target gene under the premise of chitin induction to control the production of ectoine and hydroxyectoine. We verified its regulation effect by LB fermentation.
+https://static.igem.wiki/teams/4621/wiki/parts/fermentation-with-an-inducible-promoter.png
-<!-- Add more about the biology of this part here
-===Usage and Biology===
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