Difference between revisions of "Part:BBa K4883001"

 
 
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The GTP cyclohydrolase II of Saccharomyces cerevisiae. It is the first step of its riboflavin synthesis pathway.  
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RIB1 encodes for GTP cyclohydrolase II in S. cerevisiae. This enzyme catalyzes the first step of the riboflavin biosynthesis pathway, transforming GTP to 2,5-diamino-6-(5-phospho-D-ribosylamino)-pyrimidin-4(3H)-one (DARPP) (Gudipati et al., 2014).  
  
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===Usage and Biology===
 
  
 
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===Usage and Biology===
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RIB1 is a riboflavin synthase. Overexpression of RIB1 could lead to the overproduction of vitamin B2 in S. cerevisiae.
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<html>
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<body>
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<figure>
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<div class = "center">
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<center><img src = "https://static.igem.wiki/teams/4883/wiki/part-bba-k4883001-1.png" style = "width:480px"></center>
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</div>
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<figcaption><center>Figure 1. Gel electrophoresis of colony PCR products for verification of correct transformation of plasmid pCEV-G4-RIB1-Km into E. coli DH5α </center></figcaption>
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</figure>
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</body>
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==References==
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Gudipati, V.; Koch, K.; Lienhart, W.-D.; Macheroux, P. The Flavoproteome of the Yeast Saccharomyces Cerevisiae. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 2014, 1844 (3), 535–544. DOI:10.1016/j.bbapap.2013.12.015.
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Latest revision as of 01:42, 6 October 2023


RIB1, GTP cyclohydrolase II from Saccharomyces cerevisiae

RIB1 encodes for GTP cyclohydrolase II in S. cerevisiae. This enzyme catalyzes the first step of the riboflavin biosynthesis pathway, transforming GTP to 2,5-diamino-6-(5-phospho-D-ribosylamino)-pyrimidin-4(3H)-one (DARPP) (Gudipati et al., 2014).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 428
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 448
  • 1000
    COMPATIBLE WITH RFC[1000]



Usage and Biology

RIB1 is a riboflavin synthase. Overexpression of RIB1 could lead to the overproduction of vitamin B2 in S. cerevisiae.

Figure 1. Gel electrophoresis of colony PCR products for verification of correct transformation of plasmid pCEV-G4-RIB1-Km into E. coli DH5α

References

Gudipati, V.; Koch, K.; Lienhart, W.-D.; Macheroux, P. The Flavoproteome of the Yeast Saccharomyces Cerevisiae. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 2014, 1844 (3), 535–544. DOI:10.1016/j.bbapap.2013.12.015.