Difference between revisions of "Part:BBa K233002:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | A suitable export tag was synthesizd and added in frame. the export tag was designed based on the twin arginine translocation (tat) pathway from E.coli | |
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===Source=== | ===Source=== |
Latest revision as of 10:26, 18 October 2009
Snowdrift 2 , using YcdB. A module to secrete B-galactosidase using the TAT-export Pathway
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3918
Design Notes
A suitable export tag was synthesizd and added in frame. the export tag was designed based on the twin arginine translocation (tat) pathway from E.coli
Source
source- biobricks torA,lacZ, pLac - eschercihia coli GFP- aequoria victoria