Difference between revisions of "Part:BBa K233002"
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1. A constituive promoter | 1. A constituive promoter | ||
2. RBS (ribosome binding site) | 2. RBS (ribosome binding site) | ||
| − | 3. An export tag viz. | + | 3. An export tag viz. YcdB |
4. Lac Z gene (Gene for enzyme B-galactosidase) | 4. Lac Z gene (Gene for enzyme B-galactosidase) | ||
5. A reporter gene viz. GFP | 5. A reporter gene viz. GFP | ||
Latest revision as of 10:26, 18 October 2009
Snowdrift 2 , using YcdB. A module to secrete B-galactosidase using the TAT-export Pathway
This is a module that contains the following components: 1. A constituive promoter 2. RBS (ribosome binding site) 3. An export tag viz. YcdB 4. Lac Z gene (Gene for enzyme B-galactosidase) 5. A reporter gene viz. GFP 6. TERM
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3918