Difference between revisions of "Part:BBa K4621151:Design"

 
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===References===
 
===References===
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[1]Garcia-Estepa R et al.Sys Appl Microbiol, 2006, 29 (8) :626-633

Latest revision as of 17:44, 5 October 2023


It consists of the chi18B hypothetical promoter and EctOperon.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3567
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 3567
    Illegal NotI site found at 911
    Illegal NotI site found at 1796
    Illegal NotI site found at 2114
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1093
    Illegal XhoI site found at 1267
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3567
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3567
    Illegal NgoMIV site found at 1128
    Illegal NgoMIV site found at 1534
    Illegal NgoMIV site found at 1540
    Illegal NgoMIV site found at 1831
    Illegal NgoMIV site found at 2880
    Illegal NgoMIV site found at 3072
    Illegal NgoMIV site found at 3582
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 716
    Illegal BsaI.rc site found at 896
    Illegal BsaI.rc site found at 1678
    Illegal BsaI.rc site found at 2206
    Illegal BsaI.rc site found at 2461
    Illegal BsaI.rc site found at 2551
    Illegal BsaI.rc site found at 2626
    Illegal SapI site found at 754


Design Notes

The homology arm and the GC content of the sequence were considered when designing the primers for each fragment.


Source

This part is derived from SCUT-3 genome.

References

[1]Garcia-Estepa R et al.Sys Appl Microbiol, 2006, 29 (8) :626-633