Difference between revisions of "Part:BBa K4621072"

Revision as of 16:56, 5 October 2023

Fusion promoter composed of Pro1007 and ProP

Pro1007 contains the promoter of GQS52_24880 in SCUT-3. The fusion promoter composed of Pro1007（BBa_K3880007）and ProP（BBa_K4621004）can control the gene expression switch while controlling its expression intensity. Usage, Biology and Characterization Pro1007 was inserted into the chitin-inducible promoter ProP to form a fusion promoter. The regulation of gene expression can be achieved by placing it before the coding of the target gene. In our study, Pro1007 stably expressed the target gene under the premise of chitin induction to control the production of ectoine and hydroxyectoine. We verified its regulation effect by LB fermentation.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 120
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 162
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4621072 short</partinfo>
-This CRISPRi fragment contains four parts : BBa _ K4621070, BBa _ K3875019, BBa _ K4621030, and BBa _ K3081104, covering the dCas9 and sgRNA necessary for the CRISPRi system. The CRISPRi system is suitable for a variety of Streptomyces.[1] In this study, it was used to inhibit the expression of GQS52 _ 08040 gene in SCUT-3 to produce more ectoine and hydroxyectoine.
+Pro1007 contains the promoter of GQS52_24880 in SCUT-3. The fusion promoter composed of Pro1007（BBa_K3880007）and ProP（BBa_K4621004）can control the gene expression switch while controlling its expression intensity.
 Usage, Biology and Characterization
+Pro1007 was inserted into the chitin-inducible promoter ProP to form a fusion promoter. The regulation of gene expression can be achieved by placing it before the coding of the target gene. In our study, Pro1007 stably expressed the target gene under the premise of chitin induction to control the production of ectoine and hydroxyectoine. We verified its regulation effect by LB fermentation.
-Due to the limited types of plasmids available for SCUT-3, we inserted the CRISPRi fragment into the previously constructed plasmid by homologous recombination to achieve simultaneous stable expression of multiple target genes. Subsequently, we verified the effectiveness of the CRISPRi system in the fermentation of ordinary LB, high-salt LB and shrimp shells.