Difference between revisions of "Part:BBa K4989000:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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The sequence that we designed has a start and ending codon and a single open reading frame (ORF). Also, the sequence is expected to be expressed in our hosts Lactobacillus rhamnosus GG, Lactobacillus plantarum LP-01, Lactobacillus acidophilus DSM 20079 and E.coli K-12 and thus was codon-optimized.
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The sequence that we designed has a start and ending codon and a single open reading frame (ORF). Also, the sequence is expected to be expressed in our hosts <i>Lactobacillus rhamnosus GG</i>, <i>Lactobacillus plantarum LP-01</i>, <i>Lactobacillus acidophilus</i> DSM 20079 and <i>E.coli K-12</i> and thus was codon-optimized.
  
 
===Source===
 
===Source===

Revision as of 13:54, 5 October 2023


Acetyl-CoA C-acetyltransferase (thiolase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 984
    Illegal BamHI site found at 849
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 132
    Illegal AgeI site found at 873
    Illegal AgeI site found at 907
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence that we designed has a start and ending codon and a single open reading frame (ORF). Also, the sequence is expected to be expressed in our hosts Lactobacillus rhamnosus GG, Lactobacillus plantarum LP-01, Lactobacillus acidophilus DSM 20079 and E.coli K-12 and thus was codon-optimized.

Source

Our part was synthetically produced but the sequence comes from Coprococcus sp. L2-50 DSM.

References