Difference between revisions of "Part:BBa K4583012"
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==Reference==
 
==Reference==
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[1] Shong, J., & Collins, C. H. (2013). Engineering the esaR promoter for tunable quorum sensing- dependent gene expression. ACS synthetic biology, 2(10), 568–575. https://doi.org/10.1021/sb4000433
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[2] Gu, F., Jiang, W., Mu, Y., Huang, H., Su, T., Luo, Y., Liang, Q., & Qi, Q. (2020). Quorum Sensing-Based Dual-Function Switch and Its Application in Solving Two Key Metabolic Engineering Problems. ACS synthetic biology, 9(2), 209–217. https://doi.org/10.1021/acssynbio.9b00290

Revision as of 13:39, 5 October 2023


PesaS

PesaS

Usage and Biology

QS system

Quorum sensing (QS) is a natural form of cell-cell communication that regulates the metabolic behaviour of bacteria based on changes in their local cell density. As cell density increases, signalling molecules accumulate and are sensed by QS-controlled gene expression regulators, which turn on relevant gene expression.

Esa I/R system

The Esa I/R system is quite special from traditional QS system. The EsaI/R QS system is homologous to the LuxI/R QS system and originates the maize pathogen--Pantoea stewartii subsp. stewartia. EsaR can act as both transcriptional activator and repressor. PesaR is a natural EsaR-repressed promoter, whereas PesaS is a natural EsaR-activated promoter. At low cell density (low ρ), EsaR binds to its esa box to turn off PesaR and turn on PesaS. In the presence of AHL, EsaR can bind to AHL and release from the DNA. Thus, at high cell density(high ρ), the PesaR is turned on and the PesaS is turned off[2].

Fig. 1 . schematic illustration of Esa I/R system

PesaS

PesaS is a natural EsaR-activated promoter.At low cell density, EsaR binds to its target DNA sequence (esa box) to turn off the expression of genes downstream of the PesaR promoter and turn on the expression of genes downstream of PesaS. In the presence of AHL, EsaR releases the esa box. As a result, at high cell density, PesaR is turned on and PesaS is turned off.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

[1] Shong, J., & Collins, C. H. (2013). Engineering the esaR promoter for tunable quorum sensing- dependent gene expression. ACS synthetic biology, 2(10), 568–575. https://doi.org/10.1021/sb4000433

[2] Gu, F., Jiang, W., Mu, Y., Huang, H., Su, T., Luo, Y., Liang, Q., & Qi, Q. (2020). Quorum Sensing-Based Dual-Function Switch and Its Application in Solving Two Key Metabolic Engineering Problems. ACS synthetic biology, 9(2), 209–217. https://doi.org/10.1021/acssynbio.9b00290