Difference between revisions of "Part:BBa K233004"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K233004 short</partinfo> | <partinfo>BBa_K233004 short</partinfo> | ||
| − | This contains the lacI binding sites without any of the -10 and -35 regions of the promoter.therefore it is only repressed by the binding of LacI | + | This contains the lacI binding sites without any of the -10 and -35 regions of the promoter.therefore it is only repressed by the binding of LacI. |
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| + | A completely symmetric DNA segment has been constructed that binds the lactose repressor of Escherichia coli 10- | ||
| + | fold more tightly than does the natural lactose operator sequence. This tight-binding operator is an inverted repeat of a 15-base-pair segment from the left half of the natural operator sequence. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 09:42, 18 October 2009
lac operator
This contains the lacI binding sites without any of the -10 and -35 regions of the promoter.therefore it is only repressed by the binding of LacI.
A completely symmetric DNA segment has been constructed that binds the lactose repressor of Escherichia coli 10- fold more tightly than does the natural lactose operator sequence. This tight-binding operator is an inverted repeat of a 15-base-pair segment from the left half of the natural operator sequence.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]