Difference between revisions of "Part:BBa K4621045"
 
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<partinfo>BBa_K4621045 short</partinfo>
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Pro1008 contains the promoter（BBa_K3880007） of GQS52_24880 in SCUT-3 without 5’-UTR. The fusion promoter composed of Pro1008 and chitin-inducible promoter can control the gene expression switch while controlling its expression intensity.
 
Pro1008 contains the promoter（BBa_K3880007） of GQS52_24880 in SCUT-3 without 5’-UTR. The fusion promoter composed of Pro1008 and chitin-inducible promoter can control the gene expression switch while controlling its expression intensity.
 
Usage, Biology and Characterization
 
Usage, Biology and Characterization
 
Pro1008 was inserted into the chitin-inducible promoter ProP to form a fusion promoter. The regulation of gene expression can be achieved by placing it before the coding of the target gene. In our study, Pro1008 stably expressed the target gene under the premise of chitin induction to control the production of ectoine and hydroxyectoine. We verified its regulation effect by LB fermentation.
 
Pro1008 was inserted into the chitin-inducible promoter ProP to form a fusion promoter. The regulation of gene expression can be achieved by placing it before the coding of the target gene. In our study, Pro1008 stably expressed the target gene under the premise of chitin induction to control the production of ectoine and hydroxyectoine. We verified its regulation effect by LB fermentation.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4621045 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K4621045 parameters</partinfo>
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Latest revision as of 13:08, 5 October 2023

Pro1008

Pro1008 contains the promoter（BBa_K3880007） of GQS52_24880 in SCUT-3 without 5’-UTR. The fusion promoter composed of Pro1008 and chitin-inducible promoter can control the gene expression switch while controlling its expression intensity. Usage, Biology and Characterization Pro1008 was inserted into the chitin-inducible promoter ProP to form a fusion promoter. The regulation of gene expression can be achieved by placing it before the coding of the target gene. In our study, Pro1008 stably expressed the target gene under the premise of chitin induction to control the production of ectoine and hydroxyectoine. We verified its regulation effect by LB fermentation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 135
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 177
  • 1000
    COMPATIBLE WITH RFC[1000]