Difference between revisions of "Part:BBa K4621045:Design"

 
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<partinfo>BBa_K4621045 short</partinfo>
 
<partinfo>BBa_K4621045 short</partinfo>
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Pro1008 contains the promoter（BBa_K3880007） of GQS52_24880 in SCUT-3 without 5’-UTR. The fusion promoter composed of Pro1008 and chitin-inducible promoter can control the gene expression switch while controlling its expression intensity.
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Usage, Biology and Characterization
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Pro1008 was inserted into the chitin-inducible promoter ProP to form a fusion promoter. The regulation of gene expression can be achieved by placing it before the coding of the target gene. In our study, Pro1008 stably expressed the target gene under the premise of chitin induction to control the production of ectoine and hydroxyectoine. We verified its regulation effect by LB fermentation.
  
 
<partinfo>BBa_K4621045 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4621045 SequenceAndFeatures</partinfo>

Revision as of 13:04, 5 October 2023


Pro1008 Pro1008 contains the promoter（BBa_K3880007） of GQS52_24880 in SCUT-3 without 5’-UTR. The fusion promoter composed of Pro1008 and chitin-inducible promoter can control the gene expression switch while controlling its expression intensity. Usage, Biology and Characterization Pro1008 was inserted into the chitin-inducible promoter ProP to form a fusion promoter. The regulation of gene expression can be achieved by placing it before the coding of the target gene. In our study, Pro1008 stably expressed the target gene under the premise of chitin induction to control the production of ectoine and hydroxyectoine. We verified its regulation effect by LB fermentation.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 135
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 177
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The effectiveness and GC content.


Source

This part is derived from SCUT-3 genome.

References