Difference between revisions of "Part:BBa K4665001:Design"

 
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===References===
 
===References===
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Mergulhao, F.J.M. et al. (January 8, 2005). Recombinant protein secretion in Escherichia coli. Biotechnology Advances, 23(3): 177-202. https://doi.org/10.1016/j.biotechadv.2004.11.003
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Zhu, Y., et al. (December 6, 2021). Surface display of carbonic anhydrase on Escherichia coli for CO2 capture and mineralisation. Synthetic and Systems biotechnology, 7(1): 460-473. https://doi.org/10.1016%2Fj.synbio.2021.11.008

Revision as of 12:56, 5 October 2023


INPN


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 470
    Illegal PstI site found at 592
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 470
    Illegal PstI site found at 592
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 470
    Illegal PstI site found at 592
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 470
    Illegal PstI site found at 592
    Illegal NgoMIV site found at 54
    Illegal AgeI site found at 555
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The INPN will be embedded in the outer cell membrane of E. coli. This can in turn be fused to a linker to present a protein on the outside of the bacterial cell.


Source

This ice nucleation protein is derived from Pseudomonas syringae. The codon optimized sequence for E. coli (BL21 DE3) has been derived from the research performed by Zhu et al. (2022).

References

Mergulhao, F.J.M. et al. (January 8, 2005). Recombinant protein secretion in Escherichia coli. Biotechnology Advances, 23(3): 177-202. https://doi.org/10.1016/j.biotechadv.2004.11.003

Zhu, Y., et al. (December 6, 2021). Surface display of carbonic anhydrase on Escherichia coli for CO2 capture and mineralisation. Synthetic and Systems biotechnology, 7(1): 460-473. https://doi.org/10.1016%2Fj.synbio.2021.11.008