Difference between revisions of "Part:BBa K4583012"
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PesaS | PesaS | ||
| + | ==Usage and Biology== | ||
| + | ===QS system=== | ||
| + | Quorum sensing (QS) is a natural form of cell-cell communication that regulates the metabolic behaviour of bacteria based on changes in their local cell density. As cell density increases, signalling molecules accumulate and are sensed by QS-controlled gene expression regulators, which turn on relevant gene expression. | ||
| + | ===Esa I/R system=== | ||
| + | The Esa I/R system is quite special from traditional QS system. The EsaI/R QS system is homologous to the LuxI/R QS system and originates the maize pathogen--<i>Pantoea stewartii</i> subsp. <i>stewartia</i>. EsaR can act as both transcriptional activator and repressor. PesaR is a natural EsaR-repressed promoter, whereas PesaS is a natural EsaR-activated promoter. At low cell density (low ρ), EsaR binds to its esa box to turn off PesaR and turn on PesaS. In the presence of AHL, EsaR can bind to AHL and release from the DNA. Thus, at high cell density(high ρ), the PesaR is turned on and the PesaS is turned off[2]. | ||
| + | <html> | ||
| + | <figure> | ||
| + | <img src="https://static.igem.wiki/teams/4583/wiki/esa.png"width="450" height="290"> | ||
| + | <figcaption><b>Fig. 1 </b>. schematic illustration of Esa I/R system </figcaption> | ||
| + | </figure> | ||
| + | </html> | ||
| + | ===PesaRp=== | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 12:45, 5 October 2023
PesaS
PesaS
Usage and Biology
QS system
Quorum sensing (QS) is a natural form of cell-cell communication that regulates the metabolic behaviour of bacteria based on changes in their local cell density. As cell density increases, signalling molecules accumulate and are sensed by QS-controlled gene expression regulators, which turn on relevant gene expression.
Esa I/R system
The Esa I/R system is quite special from traditional QS system. The EsaI/R QS system is homologous to the LuxI/R QS system and originates the maize pathogen--Pantoea stewartii subsp. stewartia. EsaR can act as both transcriptional activator and repressor. PesaR is a natural EsaR-repressed promoter, whereas PesaS is a natural EsaR-activated promoter. At low cell density (low ρ), EsaR binds to its esa box to turn off PesaR and turn on PesaS. In the presence of AHL, EsaR can bind to AHL and release from the DNA. Thus, at high cell density(high ρ), the PesaR is turned on and the PesaS is turned off[2].
PesaRp
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]