Difference between revisions of "Part:BBa K4768008"

Revision as of 12:33, 5 October 2023

Split T7 RNA polymerase (Nterm) conjugated to Pertuzumab with a transmembrane linker

Part for Expression of the T7 RNA polymerase (Nterm) conjugated to Pertuzumab with a transmembrane linker ZipA

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 987
Illegal XbaI site found at 40
Illegal SpeI site found at 1147
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 987
Illegal SpeI site found at 1147
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 987
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 987
Illegal XbaI site found at 40
Illegal SpeI site found at 1147
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 987
Illegal XbaI site found at 40
Illegal SpeI site found at 1147
Illegal AgeI site found at 1200
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 21
Illegal SapI.rc site found at 1836

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Introduction

**Figure 1: NT7-ZipA-Pertuzumab structure.**

The CALIPSO part BBa_K4768008 is composed of the N-terminal subunit of the T7 RNA polymerase (residues 1 to 180) fused to the anti-HER2 antibody Pertuzumab through an optimized linker sequence containing a transmembrane domain. This gene is under transcriptional control of an SP6 promoter and T7 terminator.

Construction

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Molecular Modeling

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Characterisation

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Conclusion and Perspectives

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References

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article 2 xxxxxxx

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              class="d-block"
              style="width:90%;"
-             src="https://static.igem.wiki/teams/4768/wiki/parts/part-008.png"
+             src="https://static.igem.wiki/teams/4768/wiki/parts/part-008.png">
              <figcaption class="normal"><span class="titre-image"><i><b>Figure 1: NT7-ZipA-Pertuzumab structure.</b></i></span></figcaption>
          </figure>