Difference between revisions of "Part:BBa K4767001"
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In 2023, CUG-China used this arsenic-responsive transcription system to detect arsenic. We placed <i>gfp</i> at downstream of the Pars -<i>arsR</i> system and demonstrated the As(Ⅲ) can bind the ArsR and drive the expression of <i>gfp</i>. As the figure shows, with an increase in arsenic concentrations, the fluorescence intensity increased in response. | In 2023, CUG-China used this arsenic-responsive transcription system to detect arsenic. We placed <i>gfp</i> at downstream of the Pars -<i>arsR</i> system and demonstrated the As(Ⅲ) can bind the ArsR and drive the expression of <i>gfp</i>. As the figure shows, with an increase in arsenic concentrations, the fluorescence intensity increased in response. | ||
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Figure 1. The fluorescence curves of <i>Shewanella oneidensis</i> MR1 cells with the arsenic-responsive transcription gene circuits. | Figure 1. The fluorescence curves of <i>Shewanella oneidensis</i> MR1 cells with the arsenic-responsive transcription gene circuits. | ||
Revision as of 11:19, 5 October 2023
Pars
This part is an arsenic-responsive promoter that couples with the transcriptional regulator ArsR. It is from Escherichia coli genome which can response to the ArsR protein and block the transcription of downstream genes.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
In 2023, CUG-China used this arsenic-responsive transcription system to detect arsenic. We placed gfp at downstream of the Pars -arsR system and demonstrated the As(Ⅲ) can bind the ArsR and drive the expression of gfp. As the figure shows, with an increase in arsenic concentrations, the fluorescence intensity increased in response.
Figure 1. The fluorescence curves of Shewanella oneidensis MR1 cells with the arsenic-responsive transcription gene circuits.
References
Jia X, Bu R, Zhao T, et al. Sensitive and specific whole-cell biosensor for arsenic detection[J]. Applied and Environmental Microbiology, 2019, 85(11): e00694-19.