Difference between revisions of "Part:BBa K4890015"
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<partinfo>BBa_K4890015 short</partinfo> | <partinfo>BBa_K4890015 short</partinfo> | ||
| − | + | This part is responsible to the expression of GFP gene driven by MRE in Drosophila. It consists of MRE sequence (BBa_K4890002), Hsp70 sequence (BBa_K4890004) and GFP gene (BBa_S03452). Metal response element (MRE) is derived from Drosophila melanogaster. In the cell nucleus, activated MTF-1 binds to MRE. MRE is typically located in the promoter regions associated with genes involved in the response to heavy metals. Binding of MTF-1 to MRE activates the transcription of the corresponding genes, thereby promoting heavy metal-related gene expression. GFP gene is derived from plasmid pcDNA3.1-CMV.GFP which was kept by Genetic Lab, School of Life science and Technology, Tongji University. It encodes green fluorescent protein (GFP) that emits green fluorescence when exposed to light in the blue to ultraviolet range. Hsp70 is derived from pUAST plasmid. Hsp70 is a promoter that can bind to RNA polymerase and start transcription. | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 10:42, 5 October 2023
MRE-Hsp70-GFP
This part is responsible to the expression of GFP gene driven by MRE in Drosophila. It consists of MRE sequence (BBa_K4890002), Hsp70 sequence (BBa_K4890004) and GFP gene (BBa_S03452). Metal response element (MRE) is derived from Drosophila melanogaster. In the cell nucleus, activated MTF-1 binds to MRE. MRE is typically located in the promoter regions associated with genes involved in the response to heavy metals. Binding of MTF-1 to MRE activates the transcription of the corresponding genes, thereby promoting heavy metal-related gene expression. GFP gene is derived from plasmid pcDNA3.1-CMV.GFP which was kept by Genetic Lab, School of Life science and Technology, Tongji University. It encodes green fluorescent protein (GFP) that emits green fluorescence when exposed to light in the blue to ultraviolet range. Hsp70 is derived from pUAST plasmid. Hsp70 is a promoter that can bind to RNA polymerase and start transcription.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 196
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 196
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 196
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 196
- 1000COMPATIBLE WITH RFC[1000]