Difference between revisions of "Part:BBa K4890011:Design"

 
 
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===Design Notes===
 
===Design Notes===
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We used pUAST vector system to construct pUAST-MTF-1 plasmid. pUAST vector contains UAS and Hsp70. We inserted MTF-1 gene in the pUAST vector downstream of UAS and Hsp70 by enzyme cutting and linking, and constructed pUAST-MTF-1 plasmid which was then transfected into Drosophila S2 cells. We also inserted GAL4 gene into the cells. GAL4 binds to UAS and can activate the expression of MTF-1 gene downstream of UAS-Hsp70.  
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===Source===
 
===Source===
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UAS sequence is based on BBa_K3281012.
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Hsp70 sequence is based on BBa_K4890004
  
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===References===
 
===References===

Latest revision as of 10:37, 5 October 2023


UAS-Hsp70


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 226
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 226
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 226
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 226
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used pUAST vector system to construct pUAST-MTF-1 plasmid. pUAST vector contains UAS and Hsp70. We inserted MTF-1 gene in the pUAST vector downstream of UAS and Hsp70 by enzyme cutting and linking, and constructed pUAST-MTF-1 plasmid which was then transfected into Drosophila S2 cells. We also inserted GAL4 gene into the cells. GAL4 binds to UAS and can activate the expression of MTF-1 gene downstream of UAS-Hsp70.


Source

UAS sequence is based on BBa_K3281012. Hsp70 sequence is based on BBa_K4890004


References