Difference between revisions of "Part:BBa K4890004:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | ... | + | We used pUAST vector system to construct pUAST-MTF-1 plasmid. pUAST vector contains UAS and Hsp70. We inserted MTF-1 gene in the pUAST vector downstream of UAS and Hsp70 by enzyme cutting and linking, and constructed pUAST-MTF-1 plasmid. |
| − | + | ||
===Source=== | ===Source=== | ||
| + | The source of the part will be its sequence which was retrieved from pUAST sequence. | ||
| − | |||
===References=== | ===References=== | ||
Latest revision as of 10:33, 5 October 2023
Hsp70
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 141
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 141
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 141
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 141
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used pUAST vector system to construct pUAST-MTF-1 plasmid. pUAST vector contains UAS and Hsp70. We inserted MTF-1 gene in the pUAST vector downstream of UAS and Hsp70 by enzyme cutting and linking, and constructed pUAST-MTF-1 plasmid.
Source
The source of the part will be its sequence which was retrieved from pUAST sequence.