Difference between revisions of "Part:BBa K4890003:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
......
+
We aim to construct Drosophila lines to detect heavy metals in the environment. MTF-1 is used to trigger the response of Drosophila to heavy metal ions. MTF-1 is a transcription factor that can be activated by heavy metals. In the cell nucleus of Drosophila cells, MTF-1 binds to MRE to recruit RNA polymerase, in turn increasing the expression of Mto/Mtn to detoxicate metals. We replaced Mto/Mtn with Hid (head involution defective) to visualize transcription process.  
−
 
+
  
  
 
===Source===
 
===Source===
 +
The source of the part will be its sequence which was retrieved from flybase.org.
  
−
......
 
  
 
===References===
 
===References===

Latest revision as of 10:29, 5 October 2023


Hid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 9
    Illegal XbaI site found at 1265
    Illegal PstI site found at 613
    Illegal PstI site found at 842
    Illegal PstI site found at 986
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal PstI site found at 613
    Illegal PstI site found at 842
    Illegal PstI site found at 986
    Illegal NotI site found at 27
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
    Illegal BglII site found at 21
    Illegal XhoI site found at 136
    Illegal XhoI site found at 1042
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 9
    Illegal XbaI site found at 1265
    Illegal PstI site found at 613
    Illegal PstI site found at 842
    Illegal PstI site found at 986
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 9
    Illegal XbaI site found at 1265
    Illegal PstI site found at 613
    Illegal PstI site found at 842
    Illegal PstI site found at 986
    Illegal NgoMIV site found at 971
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We aim to construct Drosophila lines to detect heavy metals in the environment. MTF-1 is used to trigger the response of Drosophila to heavy metal ions. MTF-1 is a transcription factor that can be activated by heavy metals. In the cell nucleus of Drosophila cells, MTF-1 binds to MRE to recruit RNA polymerase, in turn increasing the expression of Mto/Mtn to detoxicate metals. We replaced Mto/Mtn with Hid (head involution defective) to visualize transcription process.


Source

The source of the part will be its sequence which was retrieved from flybase.org.


References